Machine Washable Bath Mats
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Fill your washing machine with water and adjust the cycle to use the hottest water possible. High temperatures kill mold spores.
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Add ½ cup of distilled white vinegar. White vinegar will help remove the mold.
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Put the moldy bath mat in the vinegar water and shut the lid to start the cycle.
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Remove the bath mat from the washing machine.
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Put the bath mat in a dryer and adjust the heat to the highest that the bath mat can handle. If the bath mat manufacturer does not recommend machine drying the bath mat, hang it over a hanger until it is completely dry.
Non-Machine Washable Bath Mats
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Remove as much of the mold growth as possible by scrubbing the mold area with a dry toothbrush. This will flake off a lot of the mold, but will probably not remove all of it.
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Mix 1 cup water with 1 cup rubbing alcohol.
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Dab the rubbing alcohol solution onto the moldy area until you remove as much of the mold as possible.
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Mix 1 cup white vinegar with 1 cup water if mold is still present on the rug. Dab the vinegar solution over the moldy areas until you remove all of it.
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Rinse the bath mat with clear water to remove the vinegar and the rubbing alcohol solutions. Hang the bath mat to dry completely.